Pentafluorobenzenesulfonyl fluorescein is a H 2 O 2 -selective sensor that can be used to detect H 2 O 2 levels in cells. Pentafluorobenzenesulfonyl fluorescein is generally non-fluorescent, but emits fluorescence when its sulfonyl bond undergoes perhydrolysis by H 2 O 2 . Pentafluorobenzenesulfonyl fluorescein undergoes slight cleavage of its sulfonate ester bond by [Cu (phen) 2 ] 2+ , and can detect hydrogen peroxide around the ablation sites of fin tissues and keratinocytes in zebrafish larvae.
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