Thionicotinamide adenine dinucleotide (Thio-NAD) is a thione-modified analog of the coenzyme nicotinamide adenine dinucleotide (NAD + ). Thionicotinamide adenine dinucleotide can replace NAD + in enzymatic reactions and shift the absorption peak to 405 nM, enabling sensitive, real-time kinetic detection of enzyme activity, which is particularly suitable for high-sensitivity diagnostic cyclic detection systems.
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