p-Nitrophenyl α-L-fucoside (PNP α-L-Fuc) is a specific substrate of Pecten maximus α-L-fucosidase, with a K m value of 650 µM. p-Nitrophenyl α-L-fucoside serves as a substrate for ClAgl29A and ClAgl29B . Enzymatic hydrolysis of p-Nitrophenyl α-L-fucoside releases p-nitrophenol and α-L-fucose, where the latter undergoes mutarotation over time to form β-L-fucose. p-Nitrophenyl α-L-fucoside is not the preferred substrate for ClAgl29A and ClAgl29B .
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