4-Methylumbelliferyl-β-D-xylopyranoside (4-Methylumbelliferyl-β-D-xyloside) is an enzymatically hydrolyzable β-D-xylopyranoside substrate. The enzymatic hydrolysis of 4-Methylumbelliferyl-β-D-xylopyranoside is mediated by the stereochemistry-retaining β-D-xylosidase GSXynB2 via a two-step catalytic process (xylosylation followed by dexylosidation). 4-Methylumbelliferyl-β-D-xylopyranoside serves as a substrate for the β-xylosidase of Trichoderma reesei QM 9414, and its enzymatic hydrolysis process is limited by the dexylosidation step.
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