m7GpppGpG is an unmodified trinucleotide cap analog with enhancer activity. When incorporated into mRNA , it effectively enhances translational efficiency, stability, and protein expression levels of the mRNA . m7GpppGpG can serve as a standard cap analog for in vitro mRNA synthesis to support antigen generation and presentation by dendritic cells, although its expression efficiency in dendritic cells is 20-fold lower than that of the m7GpppApG cap .
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