3''-Deamino-3''-hydroxykanamycin B (NK-1012-1) is a kanamycin antibiotic . 3''-Deamino-3''-hydroxykanamycin B can be produced by S. tenebrarius . 3''-Deamino-3''-hydroxykanamycin B is effective against E. coli ATCC 25922 and E. coli CCARM 1A020, with MIC values of 128 μg/mL.
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