N-Aminofluorescein is a fluorescein hydrazide with spiro form, a highly selective and sensitive fluorescence probe for Cu 2+ . N-Aminofluorescein has no selective fluorescence response to other common metal ions, can be used for direct detection of Cu 2+ in biological systems with λex/em=495/516 nm. N-Aminofluorescein can be used to measure the concentration of copper ions in cells.
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