closed-HMRG is HMRG with closed spirocyclic structure. Ac-HMRG, an acetylated derivative of Hydroxymethyl rhodamine green, exists as a closed spirocyclic structure in aqueous solution at physiological pH, whereas HMRG itself takes an open nonspirocyclic structure. Ac-HMRG is colorless and nonfluorescent and HMRG is strongly fluorescent.
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