Ac-H-FluNox is an acetylated cell-compatible prodrug of H-FluNox ( HY-D2339 ). Ac-H-FluNox undergoes intracellular hydrolysis of its acetyl group by esterases to generate H-FluNox , which then undergoes a deoxygenation reaction with labile heme to form a fluorescent product. Ac-H-FluNox detects fluctuations of labile heme in living cells, acute labile heme release upon nitric oxide stimulation, and accumulation of labile heme following inhibition of heme export proteins.
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