REF1 peptide is a PORK1 agonist with an EC 50 of 0.028 nM in tomato. REF1 peptide binds to the extracellular domain of PORK1 , triggers receptor autophosphorylation, and drives downstream MPK3 / MPK6 activation, thereby initiating defense and regeneration signaling cascades. REF1 peptide induces callus formation, inhibits shoot regeneration upon continuous exposure, and enhances the regeneration and transformation efficiency of recalcitrant dicotyledonous and monocotyledonous crops.
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