CME-carbodiimide is a nucleic acid modification reagent. CME-carbodiimide reacts specifically with uracil and guanine residues of RNA , as well as guanine and thymine residues of denatured DNA ; it does not react with native DNA. Modification of DNA by CME-carbodiimide inhibits phosphodiester bond cleavage or DNA hydrolysis mediated by pancreatic ribonuclease, snake venom phosphodiesterase and deoxyribonuclease.
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