RDDB is a rhodamine-based turn-on fluorescent and colorimetric chemosensor selective for Mn 2+ , with a limit of detection of 5×10 -8 M (excitation wavelength: 480-550 nm; fluorescence signal collection range: >590 nm). In the presence of Mn 2+ , RDDB undergoes a spirolactam ring-opening reaction of its rhodamine hydrazide moiety, resulting in turn-on fluorescence and a color change. RDDB can be used for intracellular Mn 2+ imaging.
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