4-(6-Methyl-1,2,4,5-tetrazin-3-yl) benzoic acid is an asymmetric tetrazine derivative and a bioorthogonal reaction partner. 4-(6-Methyl-1,2,4,5-tetrazin-3-yl) benzoic acid can be conjugated with Bevacizumab ( HY-P9906 ) via EDC/NHS activation while retaining the VEGF -binding affinity of the antibody, thus constructing a targeting agent for pretargeted immuno-PET/NIRF imaging.
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