Product

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN

CAS No.：150347-59-4

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5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN Basic information

Product Name:	5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
Synonyms:	CARBOXYFLUORESCEIN DIACETATE, NHS ESTER;CFSE;5(6)-CARBOXYFLUORESCEIN DIACETATE-N-HYDROXY-SUCCINIMIDE ESTER;5(6)-CARBOXYFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER;5-CARBOXYFLUORESCEIN-NHS DIACETATE;5-CARBOXY-DI-O-ACETYLFLUORESCEIN N-SUCCINIMIDYL ESTER;5-CARBOXYFLUORESCEIN DIACETATE-N-HYDROXYSUCCINIMIDE ESTER;5-CARBOXY-FLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER
CAS:	150347-59-4
MF:	C29H19NO11
MW:	557.46
EINECS:
Product Categories:	marker;Fluorescent;Aromatics;Fluorescent Labels & Indicators;Heterocycles;Xanthene;Intermediates & Fine Chemicals;Pharmaceuticals
Mol File:	150347-59-4.mol

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN Chemical Properties

Melting point	152-154 °C(lit.)
storage temp.	-20°C
solubility	DMF: soluble
pka	6.45(at 25℃)
form	Solid
PH Range	Weak green ' uorescence (6.0) to strong green ' uorescence (7.2)
λmax	<300nm
Major Application	Diagnosis of cancer, restricting HIV replication, antiinflammatory agent, detecting bacteria, cell proliferation assays
InChIKey	QSWYNOQSGMUDQZ-UHFFFAOYSA-N

Safety Information

WGK Germany	3
F	8-10-21
HS Code	32129000

MSDS Information

Provider	Language
SigmaAldrich	English

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN Usage And Synthesis

Chemical Properties	5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is Off-white to light yellow powder
Uses	5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is a Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell.
Uses	Amine-reactive succinimidyl ester can potentially be used for long-term pH studies of live cells, producing a conjugate with the pH-sensitive properties of carboxyfluorescein.
Uses	A 5(6)-Carboxyfluorescein (C178215) derivative used as a flurorescent probe. 5(6)-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester is used to monitor lymphocyte proliferation by covalently labelling long-lived intracellular molecules with fluorescent dye.
General Description	5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.
Biochem/physiol Actions	5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN Preparation Products And Raw materials

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