Other grades of this product :
| Product Name: | PFM01 | | Synonyms: | PFM01;(5Z)-5-[(4-Hydroxyphenyl)methylene]-3-(2-methylpropyl)-2-thioxo-4-thiazolidinone;PFM01 >=98% (HPLC);(Z)-5-(4-Hydroxybenzylidene)-3-isobutyl-2-thioxothiazolidin-4-one | | CAS: | 1558598-41-6 | | MF: | C14H15NO2S2 | | MW: | 293.4 | | EINECS: | | Product Categories: | | Mol File: | 1558598-41-6.mol |
| PFM01 Chemical Properties |
| storage temp. | 2-8°C | | form | powder | | color | white to beige |
| PFM01 Usage And Synthesis |
| Biochem/physiol Actions | PFM01 is a cell-permeable N-alkylated Mirin (Sigma Cat. No. 475954) derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease activity. PFM01 targets MRE11 at a site near the dimer interface, distinct from that occupied by Mirin and PFM39 to allow disruption of the ssDNA-binding groove and selective inhibition against MRE11 endo-, but not exo-, nuclease activity. While both endonuclease and exonuclease activities are required for MRE11-mediated homologous recombination (HR) repair, only FM01 (100 μM), but not the exonuclease inhibitors Mirin (500 μM) and PFM39 (100 μM), rescues G2-phase double-strand break (DSB) repair defect in HR protein BRCA2-deficient HSC62-hTERT fibroblasts following ionizing irradiation (IR) by blocking HR initiation and thereby allowing non-homologous end joining (NHEJ) to proceed. |
| PFM01 Preparation Products And Raw materials |
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