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| APN-Azide 95% Basic information |
| APN-Azide 95% Chemical Properties |
| Melting point | 100°C | | storage temp. | 2-8°C | | form | powder |
| RIDADR | UN1325 - class 4.1 - PG 2 - Flammable solids, organic, n.o.s., HI: all |
| APN-Azide 95% Usage And Synthesis |
| Uses | APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.Standard protein labeling procedure (cysteine labeling)1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).3. Incubate at room temperature for 2 hours.4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.*Note: avoid thiol-containing buffers. |
| APN-Azide 95% Preparation Products And Raw materials |
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