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| Digoxigenin-11-UTP Basic information |
| Digoxigenin-11-UTP Chemical Properties |
| storage temp. | -20°C | | form | solution |
| Digoxigenin-11-UTP Usage And Synthesis |
| Uses | Digoxigenin-11-UTP has been used for labeling RNA probes in:- in situ hybridization
- in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
- fluorescence in situ hybridization (FISH)
| | General Description | Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%. | | Biochem/physiol Actions | Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids. |
| Digoxigenin-11-UTP Preparation Products And Raw materials |
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