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| RESORUFIN SODIUM SALT Basic information |
| Product Name: | RESORUFIN SODIUM SALT | | Synonyms: | RESORUFIN;RESORUFIN SODIUM SALT;7-HYDROXY-3H-PHENOXAZIN-3-ONE SODIUM SALT;resorufin sodium;RESORUFIN SODIUM SALT (DYE CONTENT 75%);RESORUFIN, FOR MICROSCOPY;3H-Phenoxazin-3-one, 7-hydroxy-, sodium salt;RESORUFIN, SODIUM SALT *FLUORESCENCE REFERENCE STANDARD* | | CAS: | 34994-50-8 | | MF: | C12H6NNaO3 | | MW: | 235.17 | | EINECS: | 252-310-2 | | Product Categories: | Aromatics;Heterocycles | | Mol File: | 34994-50-8.mol |
| RESORUFIN SODIUM SALT Chemical Properties |
| Melting point | >300 °C(lit.) | | storage temp. | room temp | | solubility | Methanol (Slightly), Water (Slightly) | | form | Solid | | color | Dark Purple to Black | | BRN | 174850 |
| Hazard Codes | Xi | | Risk Statements | 36/37/38 | | Safety Statements | 26-37-36 | | WGK Germany | 3 | | RTECS | SP7698000 |
| RESORUFIN SODIUM SALT Usage And Synthesis |
| Chemical Properties | Dark Purple Solid | | Uses | Resorufin is a pink fluorescent dye that is not fluorescent until activated by UV light. Resorufin is an anionic tricyclic dye. | | Uses | Resorufin has been used to determine the cytochrome P450 (CYP) 2A5 coumarin 7-hydroxylase (COH) activity changes over a broad range of lipopolysaccharide (LPS) doses. It has also been used as an internal standard for ethoxyresurufin-o-deethylase (EROD) analysis. | | General Description | Resorufin is a fluorescent indicator. | | Purification Methods | The free acid is purified by dissolving it in conc H2SO4 which on dilution precipitates resorufin and is filtered off, washed well with hot H2O and dried. [Eichler J Prakt Chem 139 113 1934.] Detailed UV and IR studies were reported by Musso & Mattheis Chem Ber 90 1814 1957]. [pKa: Musso & Rathjen Chem Ber 92 751 1959, Beilstein 27 1V 2263.]
The monoacetate [1152-14-3] M 252.2, m 2 2 3o is a fluorogenic substrate used for hydrolytic enzymes, and its fluorescence has Emax 593nm (Eex 500nm, in 0.1M phosphate pH 8.0 with lipase) [Kitson & Kitson Biochem J 322 701 1997]. [Beilstein 27 IV 2263.]
The O7-methylresorufin (7-methoxy-3H-phenoxazin-3-one) [5735-89-3] M 227.2, m > 2 2 0o has fluorescence at Emax 585nm (Eexcit 571 nm in deacylase solution) and is used to differentiate isoenzymes of cytochrome P-450. It is insoluble in H2O and dilute alkali but is soluble in EtOH and CHCl3 to give an orange yellow colour. [Kehrmann Justus Liebigs Ann Chem 372 352 1910, Beilstein 27 II 108.] |
| RESORUFIN SODIUM SALT Preparation Products And Raw materials |
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