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| 2′,7′-Dichlorodihydrofluorescein Diacetate Basic information |
| Product Name: | 2′,7′-Dichlorodihydrofluorescein Diacetate | | Synonyms: | 187263;2,7-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem;2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate;2',7'-DICHLOROFLUORESCIN DIACETATE, FOR FLUORESCENCE;2',7'-Dichlorodihydrofluoresceinediacetate;Dichlorofluorescin diacetate;2'',7''-Dichlorofluorescein diac;2-(2,7-Dichloro-3,6-diacetoxy-9H-xanthen-9-yl)benzoic acid | | CAS: | 4091-99-0 | | MF: | C24H16Cl2O7 | | MW: | 487.29 | | EINECS: | 685-843-8 | | Product Categories: | | Mol File: | 4091-99-0.mol |
| 2′,7′-Dichlorodihydrofluorescein Diacetate Chemical Properties |
| Melting point | 209-210 °C(lit.) | | Boiling point | 593.5±50.0 °C(Predicted) | | density | 1.463±0.06 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | DMF: soluble | | form | Crystalline solid | | pka | 3.79±0.36(Predicted) | | BRN | 4610273 | | EPA Substance Registry System | 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0) |
| 2′,7′-Dichlorodihydrofluorescein Diacetate Usage And Synthesis |
| Description | DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M−1cm−1). | | Chemical Properties | white to yellow-beige or pale pink powder | | Uses | Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide. | | Uses | 2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation. | | Uses | 2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay. | | General Description | Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress. | | Biochem/physiol Actions | Cell permeable: yes |
| 2′,7′-Dichlorodihydrofluorescein Diacetate Preparation Products And Raw materials |
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