GLUTATHIONE S-TRANSFERASE

GLUTATHIONE S-TRANSFERASE
  • CAS No.:50812-37-8
Other grades of this product :
GLUTATHIONE S-TRANSFERASE Basic information
Product Name:GLUTATHIONE S-TRANSFERASE
Synonyms:RX: GLUTATHIONE R-TRANSFERASE;PURIFIED GST;GultathioneStransferases, ≥25 units/mg protein;glutathione S-transferase from*bovine liver;GLUTATHIONE S-TRANSFERASE FROM*PORCINE L IVER;GLUTATHIONE S-TRANSFERASE FROM RAT*LIVER;GLUTATHIONE S-TRANSFERASE ISOZYME P1-1;GLUTATHIONE S-TRANSFERASE M1-1 ISOZYME
CAS:50812-37-8
MF:
MW:0
EINECS:
Product Categories:
Mol File:Mol File
GLUTATHIONE S-TRANSFERASE Chemical Properties
storage temp. -70°C
form lyophilized powder
Safety Information
Hazard Codes B
WGK Germany 3
MSDS Information
ProviderLanguage
SigmaAldrich English
GLUTATHIONE S-TRANSFERASE Usage And Synthesis
UsesGlutathione S-Transferase from human placenta has been used in antigen microarray and informatics analysis.
General DescriptionGlutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. These isozymes differ in their catalytic activity, as well as in their non-catalytic binding properties. Cytoplasmic isoforms of GST are encoded by five genes, namely α, θ, μ, σ and π. α, μ and π are the most abundant forms in mammals. Membrane bound GST forms are encoded by a single gene.
Biochem/physiol ActionsGlutathione S-transferase (GST) catalyzes conjugation reactions during phase II metabolism. This enzyme also confers protection against catabolic products of peroxidized lipid and oxidized DNA, and ROS (reactive oxygen species) or electrophiles.
Purification MethodsIt is purified by affinity chromatography using a column prepared by coupling glutathione to epoxy-saturated Sepharose. After washing contaminating proteins, the pure transferase is eluted with buffer containing reduced glutathione. The solution is then concentrated by ultrafiltration, dialysed against phosphate buffer at pH ~7 and stored in the presence of dithiothreitol (2mM) in aliquots at
GLUTATHIONE S-TRANSFERASE Preparation Products And Raw materials

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