Other grades of this product :
| COELENTERAZINE H Basic information |
| COELENTERAZINE H Chemical Properties |
| Melting point | >200℃ | | Boiling point | 593.5±60.0 °C(Predicted) | | density | 1.26±0.1 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | Soluble in methanol, ethanol | | pka | 10.50±0.30(Predicted) | | form | solid | | color | Yellow | | λmax | 437nm | | Stability: | Stable for 1 year from ate of purchase as supplied. Solutions in DMSO or methanol may be stored at -20°C for up to 1 month. | | Biological Applications | Calcium indicator; assaying luminescent enzyme; measuring luciferase activity; as a substrate for luciferase; screening HIV-1 protease inhibitors; bioluminescence resonance energy transfer (BRET) detection system |
| Hazard Codes | Xi | | Risk Statements | 36/37/38 | | Safety Statements | 26-36 | | WGK Germany | 3 | | HS Code | 2933998090 |
| COELENTERAZINE H Usage And Synthesis |
| Description | Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells. | | Uses | Coelenterazine h, is a derivative of Coelenterazine (C636000), and iis more sensitive to Ca2+ than is the native complex, thus providing a valuable tool for measuring small changes in Ca2+ concentrations. | | General Description | Coelenterazine is an imidazopyrazinone derivative. It is a substrate for bioluminescence in many luminous marine organisms. | | Biochem/physiol Actions | 10 times higher luminescence intensity than native coelenterazine. | | References | 1) Shimomura?et al. (1989),?Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J.,?261?913
2) Nakajima-Shimada?et al.?(1991),?Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA,?88?6878
3) Inouye?et al. (1992),?Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem.,?201?114 |
| COELENTERAZINE H Preparation Products And Raw materials |
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