Other grades of this product :
| HIRUDIN Basic information |
| HIRUDIN Chemical Properties |
| storage temp. | 2-8°C | | form | lyophilized powder | | color | white | | Merck | 13,4737 |
| Safety Statements | 22-24/25 | | WGK Germany | 3 | | RTECS | MR8440000 | | F | 10-21 | | HS Code | 3504009000 |
| HIRUDIN Usage And Synthesis |
| Description | Refludan was launched in Germany for heparin-associated
thrombocytopenia. Originally isolated from the saliva of leeches (Hirudo medicinalis),
the gene for recombinant hirudin was synthesized and expressed in Saccharomyces
cerevisiae. The isolated protein is 65 amino acids differing from the native protein by
the first two amino acids (Leu1 and Thr2) and Tyr63 is not sulfated, while it does retain
the three disulfide bonds. Refludan is a potent, specific and almost irreversible
inhibitor of thrombin with which it forms a 1:1 complex. The N-terminal domain binds
to the active catalytic site of thrombin, while the C-terminal end binds to the anion
binding exosite (fibrinogen binding cleft). It has several advantages over heparin: it
can inhibit thrombin bound to extracellular matrices, does not require antithrombin Ⅲ
as a cofactor, is not inhibited by activated platelet nor increases platelet activity, and is
a pure, single compound with a single mechanism of action. It is useful for myocardial
infarcts, unstable angia and cardiovascular events. Heparin treatment can be inhibited
by platelets activated by the Fc fragment of antibodies developed to the antigen of
heparin-platelet factor 4. A corresponding inactivation of Refuldan is not known. | | Originator | Hoechst Merion Roussel (Germany) | | Uses | Recombinant hirudin from yeast has been used in mannan-binding lectin serine protease 2 (MASP2) activation assay. It has also been used in pre-treating the nanosheets in phosphate buffered saline (PBS) for platelet adhesion assay. | | Brand name | Refludan | | General Description | Hirudin is a 7 kDa acidic protein containing 65 amino acid residues that has an isoelectric point of 3.5-4.0. It is not glycosylated and lacks tryptophan, arginine and methionine residues. It is chemically stable between pH 2-12 and at temperatures up to 80°C. | | Biochem/physiol Actions | The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It forms a high-affinity 1:1 complex with thrombin, occluding both the proteolytic site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation, and has been suggested as the clinically preferred anticoagulant (over heparin and citrate) for its specific mode of action and absence of side effects. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration. |
| HIRUDIN Preparation Products And Raw materials |
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