Other grades of this product :
| DPN I Chemical Properties |
| storage temp. | -20°C | | form | buffered aqueous glycerol solution |
| RIDADR | UN3373 - class 6.2 Biological substance, Category B | | WGK Germany | 3 |
| DPN I Usage And Synthesis |
| Uses | In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR. | | General Description | IsoschizomersThe enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.Methylation sensitivityThe enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.Typical ligation and recutting assayDpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis. |
| DPN I Preparation Products And Raw materials |
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