Other grades of this product :
| PROTAMINE SULFATE GRADE III FROM HERRING Basic information |
| Product Name: | PROTAMINE SULFATE GRADE III FROM HERRING | | Synonyms: | PROTAMINE SULFATE GRADE III FROM HERRING;protamine sulfate from herring;Protamine sulfate salt herring;clupeine;protamine sulfate salt from herring;PROTAMINE SULFATE GRADE III FROM HERRING USP/EP/BP | | CAS: | 9007-31-2 | | MF: | | MW: | 0 | | EINECS: | 232-726-0 | | Product Categories: | | Mol File: | Mol File |
| PROTAMINE SULFATE GRADE III FROM HERRING Chemical Properties |
| pka | 7.4-8.0; pKb 2.9-3.3(at 25℃) | | Merck | 13,2449 |
| WGK Germany | 3 | | RTECS | UK9450000 | | F | 21-10 |
| PROTAMINE SULFATE GRADE III FROM HERRING Usage And Synthesis |
| Uses | May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer. | | Biochem/physiol Actions | Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1. | | Purification Methods | Protamine sulfate is a strongly basic protein (white powder, see pK) used to precipitate nucleic acids from crude protein extracts. It dissolves to the extent of 1.25% in H2O. It is freely soluble in hot H2O but separates as an oil on cooling. It has been purified by chromatography on an IRA-400 ion-exchange resin in the SO 4 form and is washed with dilute H2SO4. Eluates are freeze-dried under high vacuum below 20o. This method is used to convert proteamine and protamine hydrochloride to the sulfate. [UV: Rasmussen Hoppe Seyler's Z Physiol Chem 224 97 1934, Ando & Sawada J Biochem (Tokyo) 49 252 1961, Felix & Hashimoto Hoppe Seyler's Z Physiol Chem 330 205 1963.] |
| PROTAMINE SULFATE GRADE III FROM HERRING Preparation Products And Raw materials |
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