Acetyl-CoA synthetase

Acetyl-CoA synthetase
  • CAS No.:9012-31-1
Other grades of this product :
Acetyl-CoA synthetase Basic information
Product Name:Acetyl-CoA synthetase
Synonyms:S-acetyl coenzyme A synthetase*from bakers yeast;Synthetase, acetyl coenzyme A;acetate coa ligase (amp forming);s-acetyl-coenzyme a synthetase from baker's yeast (s. cerevisiae);Acetate CoA ligase (AMP forming), Acetate thiokinase;ACETYL-COA SYNTHETASE;ACETATE: COA LIGASE;ACETATE THIOKINASE
CAS:9012-31-1
MF:
MW:0
EINECS:232-729-7
Product Categories:
Mol File:Mol File
Acetyl-CoA synthetase Chemical Properties
storage temp. -20°C
form lyophilized powder
Safety Information
WGK Germany 3
MSDS Information
ProviderLanguage
SigmaAldrich English
Acetyl-CoA synthetase Usage And Synthesis
UsesS-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO2 fixation. It may also be used in gene expression studies.
Biochem/physiol ActionsAcetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol .
Purification MethodsThis enzyme has been purified by extraction into phosphate buffer pH 6.8-7.0 containing 2-mercaptoethanol and EDTA, protamine sulfate precipitation, polyethylene glycol fractionation, Alumina gel filtration, concentration by (NH4)2SO4 precipitation, Bio-Gel A-0.5m chromatography and DEAE-cellulose gradient chromatography. It has Km (apparent) 0.24mM (for acetate) and 0.035mM (for CoA); 1.2 mM for ATP and Mg2+ 4.0mM. [Frenkel & Kitchens Methods Enzymol 71 317 1981.]
Acetyl-CoA synthetase Preparation Products And Raw materials

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