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| ENOLASE Basic information |
| Product Name: | ENOLASE | | Synonyms: | 2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase;Enolase from baker's yeast (S. cerevisiae),2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase;Enolase, 2-phospho-D-glycerate hydro-lyase;Phosphopyruvate hydratase (from Yeast), 50% glycerol solution;ENOLASE;EC 4.2.1.11;EC: 4.2.1.11;2-PHOSPHO-D-GLYCERATE HYDRO-LYASE | | CAS: | 9014-08-8 | | MF: | NULL | | MW: | 0 | | EINECS: | 232-754-3 | | Product Categories: | enzyme | | Mol File: | Mol File |
| ENOLASE Chemical Properties |
| Hazard Codes | B | | WGK Germany | 3 |
| ENOLASE Usage And Synthesis |
| Uses | The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis. | | Definition | An enzyme active in glycolysis that catalyzes the conversion of 2-phosphoglyceric acid to the phosphorylated enol of pyruvic acid. | | General Description | Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg2+ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes. | | Biochem/physiol Actions | Enolase plays a key role in glycolysis and gluconeogenesis. Enolase, found in the nucleus of mammalian cells plays a key role in transcriptional modulation of genes, which participates in morphological transformation and cell proliferation. |
| ENOLASE Preparation Products And Raw materials |
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