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| CLOSTRIPAIN Basic information |
| Product Name: | CLOSTRIPAIN | | Synonyms: | ENDOPROTEINASE-ARG-C;EC 3.4.22.8;CLOSTRIPAIN;CLOSTRIDIOPEPTIDASE B;IUB: 3.4.22.8;PROTEINASE;clostripain from clost. histolyticum;CLOSTRIPAIN FROM CLOST. HISTOLYTICUM >1 | | CAS: | 9028-00-6 | | MF: | | MW: | 0 | | EINECS: | 232-822-2 | | Product Categories: | | Mol File: | Mol File |
| CLOSTRIPAIN Chemical Properties |
| storage temp. | 2-8°C | | form | lyophilized powder | | Water Solubility | Soluble in water |
| CLOSTRIPAIN Usage And Synthesis |
| Uses | A protease that cleaves proteins on the carboxyl bond of arginine | | Uses | Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43). | | Biochem/physiol Actions | Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds. | | Purification Methods | Clostripain is isolated from Clostridium histolyticum callogenase by extraction in pH 6.7 buffer, followed by hydroxylapatite chromatography with a 0.1-0.2 M phosphate gradient, then Sephadex G-75 gel filtration with 0.05M phosphate pH 6.7, dialysis and a second hydroxylapatite chromatography (gradient elution with 0.1M 0.3M phosphate, pH 6.7). It has proteinase and esterase activity and is assayed by hydrolysing N-benzoyl-L-arginine methyl ester. [Mitchell & Harrington J Biol Chem 243 4683 1968, Methods Enzymol 19 635 1970.] |
| CLOSTRIPAIN Preparation Products And Raw materials |
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