CHOLINE OXIDASE

CHOLINE OXIDASE
  • CAS No.:9028-67-5
Other grades of this product :
CHOLINE OXIDASE Basic information
Product Name:CHOLINE OXIDASE
Synonyms:EC 1.1.3.17;CHOLINE OXIDASE;CHOLINE OXYGEN 1-OXIDOREDUCTASE;CHOLINE: OXYGEN 1-OXIDOREDUCTASE;choline oxidase from alcaligenes sp.;choline oxidase from arthrobacter*globiformis;Oxidase, choline;CHOLINE OXIDASE FROM ALCALIGENESSPECIES
CAS:9028-67-5
MF:NULL
MW:0
EINECS:232-840-0
Product Categories:
Mol File:Mol File
CHOLINE OXIDASE Chemical Properties
storage temp. -20°C
form powder
color yellow
Safety Information
Safety Statements 22-24/25
WGK Germany 3
10-21
MSDS Information
ProviderLanguage
SigmaAldrich English
CHOLINE OXIDASE Usage And Synthesis
UsesCholine oxidase from Alcaligenes sp. has been used in a study to investigate a choline biosensor constructed with chitinous membrane and its application in measuring cholinesterase inhibitory activities. Choline oxidase from Alcaligenes sp. has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin.
UsesCholine oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.
Biochem/physiol ActionsCholine oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5.
CHOLINE OXIDASE Preparation Products And Raw materials

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