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| EC 3.4.22.1 Basic information |
| Product Name: | EC 3.4.22.1 | | Synonyms: | CATHEPSIN B;CATHEPSIN B1, BOVINE SPLEEN;CATHEPSIN B, BOVINE SPLEEN;EC 3.4.22.1;CATHEPSIN B FROM HUMAN LIVER;CathepsinB from human placenta,CathepsinB1;CathepsinB from bovine spleen,Cathepsin B1;CATHEPSIN B FROM HUMAN PLACENTA | | CAS: | 9047-22-7 | | MF: | NULL | | MW: | 0 | | EINECS: | | Product Categories: | | Mol File: | Mol File |
| EC 3.4.22.1 Chemical Properties |
| storage temp. | -70°C | | solubility | H2O: soluble1.0mg/mL, clear | | form | lyophilized powder |
| Hazard Codes | B | | WGK Germany | 3 | | RTECS | FI5725000 | | F | 10-21 |
| EC 3.4.22.1 Usage And Synthesis |
| Uses | Cathepsin B from human liver has been used:- as a positive control in the screening Cathepsin B inhibitor assay
- in wall extension (expansin activity) assay
- in microinjection human foreskin fibroblasts to test its effect on apoptosis induction
| | Uses | Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells. | | General Description | Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal. | | Biochem/physiol Actions | Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis. It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption. | | Purification Methods | Cathepsin B is purified by affinity chromatography on the semicarbazone of Gly-Phe-glycinal-linked to Sepharose 4B, with elution by 2,2'-dipyridyl disulfide [Rich et al. Biochem J 235 731 1986, Methods Enzymol 80 551 1981]. |
| EC 3.4.22.1 Preparation Products And Raw materials |
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