LAMBDA PHAGE DNA

LAMBDA PHAGE DNA
  • CAS No.:91080-14-7
Other grades of this product :
LAMBDA PHAGE DNA Basic information
Product Name:LAMBDA PHAGE DNA
Synonyms:PHAGE LAMBDA DEOXYRIBONUCLEIC ACID;PHAGE LAMBDA DNA;DEOXYRIBONUCLEIC AC. BACTERIOPH. (LAMB&;DEOXYRIBONUCLEIC ACID BACTERIOPHAGE*FROM LAMBDA PHA;Deoxyribonucleic acid methylated, bacteriophage (Lambda Phage) from E.coli;DEOXYRIBONUCLEIC ACID, BACTERIOPHAGELAMB DA PHAGE A;DEOXYRIBONUCLEIC ACID, BACTERIOPHAGELAMB DA PHAGE;lambda phage dna,methylated from escherichia coli host strain w3110
CAS:91080-14-7
MF:
MW:0
EINECS:293-505-2
Product Categories:
Mol File:Mol File
LAMBDA PHAGE DNA Chemical Properties
storage temp. -20°C
form lyophilized powder
Safety Information
Risk Statements 36/37/38
Safety Statements 22-24/25-26
WGK Germany 3
3-10-21
MSDS Information
ProviderLanguage
SigmaAldrich English
LAMBDA PHAGE DNA Usage And Synthesis
UsesLambda Phage DNA, Non-methylated from Escherichia coli host strain GM119 (rm-,dam-,dcm-) has been used:
  • to calibrate spectrophotometer (LKB) and HPLC (high performance liquid chromatography) method for determination of bacterial genomic DNA G+C content
  • to amplify the λ; exonuclease gene by polymerase chain reaction (PCR)
  • in DNase activities
UsesLambda Phage DNA, Methylated from Escherichia coli host strain W3110 is suitable for use as a substrate for restriction enzymes. It was used for immunoprecipitation in ChIP assays.
General DescriptionThe DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
LAMBDA PHAGE DNA Preparation Products And Raw materials

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