5,5′-Dithiobis(2-nitrobenzoic acid)

CAS No.：69-78-3

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5,5′-Dithiobis(2-nitrobenzoic acid) Basic information

Product Name:	5,5′-Dithiobis(2-nitrobenzoic acid)
Synonyms:	DTNB;ellmann's reagent;ELLMAN'S REAGENT;ELLMANS' REAGENT;BIS(3-CARBOXY-4-NITROPHENYL) DISULFIDE;3-CARBOXY-4-NITROPHENYL DISULFIDE;Ellmsn'sreagent;Bis(3-carboxy-4-nitrophenyl) disulphide~3-Carboxy-4-nitrophenyl disulphide~Ellmans
CAS:	69-78-3
MF:	C14H8N2O8S2
MW:	396.35
EINECS:	200-714-4
Product Categories:	marker;Phenyls & Phenyl-Het;Phenyls & Phenyl-Het
Mol File:	69-78-3.mol

5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties

Melting point	240-245 °C (dec.)(lit.)
Boiling point	671.9±55.0 °C(Predicted)
density	1.67
refractive index	1.6900 (estimate)
storage temp.	Keep in dark place,Sealed in dry,Room Temperature
solubility	Do you have solubility information on this product that you would like to share
form	Yellow solid
pka	1.57±0.25(Predicted)
color	Pale Yellow to Light Yellow
Water Solubility	Soluble in water and ethanol.
Merck	14,3551
BRN	1896821
Stability:	Stable. Incompatible with strong bases, strong oxidizing agents.
InChIKey	KIUMMUBSPKGMOY-UHFFFAOYSA-N
CAS DataBase Reference	69-78-3(CAS DataBase Reference)
EPA Substance Registry System	Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3)

Safety Information

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
WGK Germany	3
RTECS	DG9650000
F	10
TSCA	Yes
HS Code	29309070

MSDS Information

Provider	Language
3-Carboxy-4-nitrophenyl disulfide	English
SigmaAldrich	English
ALFA	English

5,5′-Dithiobis(2-nitrobenzoic acid) Usage And Synthesis

Chemical Properties	Crystalline
Uses	5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample.
Uses	Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content.
Uses	5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.
Definition	ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups.
General Description	5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS^-) reacts with Ellman′s reagent (DTNB^2-), to produce 2-nitro-5-thiobenzoate anion (TNB^2-) and one mixed disulfide (R-S-TNB^-). TNB^2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.

5,5′-Dithiobis(2-nitrobenzoic acid) Preparation Products And Raw materials

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