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| 5-BROMO-4-CHLORO-3-INDOLYL B-D- Basic information |
| Product Name: | 5-BROMO-4-CHLORO-3-INDOLYL B-D- | | Synonyms: | 5-BROMO-4-CHLORO-3-INDOLYL-β-D-GLUCORONIDE CYCLOHEXYLAMMONIUM SALT;5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide;5-Bromo-4-chloro-3-indoxyl-β-D-glucuronic acid, cyclohexylammonium salt monohydrate;5-BroMo-4-chloro-1H-indol-3-yl β-D-Glucopyranosiduronic Acid MonosodiuM Salt;5-Bromo-4-chloro-3-indolyl beta-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt
X-Gluc CHA Salt
X-GlcA CHA Salt;X-gluc/X-GlcA;5-BroMo-4-chloro-3-indolyl β-D-Glucuronide CyclohexylaMMoniuM Salt [for BiocheMical Research];5-BroMo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylaMMoniuM salt hydrate (X-GLUC) | | CAS: | 114162-64-0 | | MF: | C20H26BrClN2O7 | | MW: | 521.79 | | EINECS: | 601-302-0 | | Product Categories: | substrate;Carbohydrates & Derivatives;Fluorescent Labels & Indicators;Glucuronides;Labeling and Diagnostics Reagents | | Mol File: | 114162-64-0.mol |
| 5-BROMO-4-CHLORO-3-INDOLYL B-D- Chemical Properties |
| 5-BROMO-4-CHLORO-3-INDOLYL B-D- Usage And Synthesis |
| Chemical Properties | White Solid | | Uses | 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt has been used as a histochemical reagent in X-GLUC staining. | | Uses | Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.X-GlcA has been used in histochemical staining of root sections for light microscopic observation. | | General Description | X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. | | Biochem/physiol Actions | 5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA) is a colorless substrate. The pre-immobilized X-GlcA can be converted by β-glucuronidase enzyme, synthesised by?Escherichia coli. |
| 5-BROMO-4-CHLORO-3-INDOLYL B-D- Preparation Products And Raw materials |
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