5-BROMO-4-CHLORO-3-INDOLYL B-D-

5-BROMO-4-CHLORO-3-INDOLYL B-D-
  • CAS No.:114162-64-0
Other grades of this product :
5-BROMO-4-CHLORO-3-INDOLYL B-D- Basic information
Product Name:5-BROMO-4-CHLORO-3-INDOLYL B-D-
Synonyms:5-BROMO-4-CHLORO-3-INDOLYL-β-D-GLUCORONIDE CYCLOHEXYLAMMONIUM SALT;5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide;5-Bromo-4-chloro-3-indoxyl-β-D-glucuronic acid, cyclohexylammonium salt monohydrate;5-BroMo-4-chloro-1H-indol-3-yl β-D-Glucopyranosiduronic Acid MonosodiuM Salt;5-Bromo-4-chloro-3-indolyl beta-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt X-Gluc CHA Salt X-GlcA CHA Salt;X-gluc/X-GlcA;5-BroMo-4-chloro-3-indolyl β-D-Glucuronide CyclohexylaMMoniuM Salt [for BiocheMical Research];5-BroMo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylaMMoniuM salt hydrate (X-GLUC)
CAS:114162-64-0
MF:C20H26BrClN2O7
MW:521.79
EINECS:601-302-0
Product Categories:substrate;Carbohydrates & Derivatives;Fluorescent Labels & Indicators;Glucuronides;Labeling and Diagnostics Reagents
Mol File:114162-64-0.mol
5-BROMO-4-CHLORO-3-INDOLYL B-D- Chemical Properties
Melting point 228-230°C
alpha -87~-94°(20℃/D)(c=1,H2O-DMF)(calculated on the dehydrous basis)
density 1.4241 (rough estimate)
refractive index 1.6110 (estimate)
storage temp. -20°C
solubility DMF: soluble
form tablet
color White to off-white or colorless
Stability:Hygroscopic
InChIKeyPSBARFROSMJLJR-OXLZRBRISA-N
EPA Substance Registry System.beta.-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1) (114162-64-0)
Safety Information
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-37/39-24/25
WGK Germany 3
8-10-21
HS Code 29349990
MSDS Information
5-BROMO-4-CHLORO-3-INDOLYL B-D- Usage And Synthesis
Chemical PropertiesWhite Solid
Uses5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt has been used as a histochemical reagent in X-GLUC staining.
UsesSuitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.X-GlcA has been used in histochemical staining of root sections for light microscopic observation.
General DescriptionX-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Biochem/physiol Actions5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA) is a colorless substrate. The pre-immobilized X-GlcA can be converted by β-glucuronidase enzyme, synthesised by?Escherichia coli.
5-BROMO-4-CHLORO-3-INDOLYL B-D- Preparation Products And Raw materials

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