Other grades of this product :
| Product Name: | Bicine | | Synonyms: | n,n-dihydroxyethyl-glycin;Bicine
N,N-Bis(2-hydroxyethyl)glycine
Diethanolglycine;N,N-DI(2-HYDROXYETHYL)GLYCINE;N,N-BIS(2-HYDROXYETHYL)GLYCINE;N N-DI(HYDROXYETHYL)-B-AMINOACETIC ACID;BICINE[N,N-Di(2-hydroxyethyl)glycine];BICIN (N,N-Bis-(2-hydroxyethyl)-glycin);N,N-Di(2-hydroxyethyl)glycine  | | CAS: | 150-25-4 | | MF: | C6H13NO4 | | MW: | 163.17 | | EINECS: | 205-755-1 | | Product Categories: | Biochemistry;Good's Buffers;Buffer | | Mol File: | 150-25-4.mol |
| Bicine Chemical Properties |
| Melting point | 190 °C (dec.)(lit.) | | Boiling point | 290.25°C (rough estimate) | | density | 1.05 g/mL at 20 °C | | vapor pressure | 0.002-0.007Pa at 20-25℃ | | refractive index | 1.4240 (estimate) | | storage temp. | room temp | | solubility | H2O: 1 M at 20 °C, clear, colorless | | form | Powder | | color | White | | PH | 4.0-5.5 (25℃, 1M in H2O) | | pka | 8.3(at 25℃) | | PH Range | 7.6 - 9.0 | | Water Solubility | soluble | | λmax | λ: 260 nm Amax: 0.070λ: 280 nm Amax: 0.040 | | Merck | 14,1201 | | BRN | 1769362 | | LogP | 2.3 at 20℃ and pH7 | | Surface tension | 72.2mN/m at 1g/L and 20℃ | | CAS DataBase Reference | 150-25-4(CAS DataBase Reference) | | EPA Substance Registry System | Bicine (150-25-4) |
| Bicine Usage And Synthesis |
| Chemical Properties | White/clear crystalline powder | | Uses | To prepare stable substrate solution for the determination of guanine deaminase. The buffer is widely used with the working concentration of 3-100mM. | | Uses | Biological buffer and chelating agent. | | Uses | A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described. | | Purification Methods | Dissolve bicine in a small volume of hot water and precipitate it with EtOH, twice. Repeat once more but treat the aqueous solution with charcoal Purification of Biochemicals — Amino Acids and Peptides and filter before adding EtOH. Also crystallise it from concentrated aqueous solutions. [Torn & Kolthoff J Am Chem Soc 77 2061 1955, Chaberek et al. J Am Chem Soc 75 2185 1953, Beilstein 4 IV 2390.] |
| Bicine Preparation Products And Raw materials |
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