| 5(6)-Carboxyfluorescein Basic information |
| Product Name: | 5(6)-Carboxyfluorescein |
| Synonyms: | 9’-[9h]xanthene]-ar-carboxylicacid,3’,6’-dihydroxy-spiro[isobenzofuran-1(3h;5-(AND-6)-CARBOXYFLUORESCEIN;5(6)-FAM;5(6)-CARBOXYFLUORESCEIN;4(5)-CARBOXYFLUORESCEIN;3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid;5(6)-CARBOXYFLUORESCEIN, FOR FLUORESCENC E;5(6)-CARBOXYFLUORESCEIN 99% |
| CAS: | 72088-94-9 |
| MF: | C42H24O14 |
| MW: | 752.63 |
| EINECS: | 276-331-1 |
| Product Categories: | marker;Fluorescent Labels & Indicators;Electronic Chemicals |
| Mol File: | 72088-94-9.mol |
| 5(6)-Carboxyfluorescein Chemical Properties |
| Melting point | 275 °C |
| Boiling point | 464.9°C (rough estimate) |
| density | 1.3418 (rough estimate) |
| refractive index | 1.6380 (estimate) |
| storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
| solubility | DMSO (Sparingly), Methanol (Slightly, Heated) |
| form | Solid |
| color | Light Yellow to Dark Brown |
| Water Solubility | slightly soluble |
| InChIKey | CPRVUBZYHZGASQ-UHFFFAOYSA-N |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9) |
| Safety Information |
| Hazard Codes | Xi,Xn |
| Risk Statements | 36-36/37/38-20/21/22 |
| Safety Statements | 37/39-26-36-22 |
| WGK Germany | 3 |
| TSCA | Yes |
| HS Code | 29322090 |
| MSDS Information |
| Provider | Language |
|---|---|
| 5(6)-Carboxyfluorescein | English |
| ACROS | English |
| ALFA | English |
| 5(6)-Carboxyfluorescein Usage And Synthesis |
| Description | 5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers. |
| Chemical Properties | Dark-Orange to Brown Solid |
| Uses | A popular probe of determination of intracellular pH. |
| Uses | 5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. |
| Uses | As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent |
| 5(6)-Carboxyfluorescein Preparation Products And Raw materials |
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