4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE

4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
  • CAS No.:333338-18-4
Other grades of this product :
4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE Basic information
Product Name:4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
Synonyms:PNP-PHOS DINA HEXAHYDRATE;PNPP DISODIUM HEXAHYDRATE;PNPP DISODIUM SALT HEXAHYDRATE;PHOSPHATASE SUBSTRATE;PHOSPHATASE SUBSTRATE DISODIUM SALT HEXAHYDRATE;p-Nitrophenyl phosphate disodium hexahydrate ,98%;Disodium 4-Nitrophenyl Phosphate Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt;SodiuM 4-nitrophenyl phosphate hexahydrate
CAS:333338-18-4
MF:C6H16NNa2O12P
MW:371.14
EINECS:224-246-5
Product Categories:substrate
Mol File:333338-18-4.mol
4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE Chemical Properties
Melting point >300 °C(lit.)
storage temp. -20°C
solubility H2O: 25 mg/mL at 20 °C, clear, yellow-green
form tablets
color white to pale yellow
PHpH (50g/l, 25℃) : 8.0~9.0
Water Solubility almost transparency
BRN 5324661
CAS DataBase Reference333338-18-4(CAS DataBase Reference)
Safety Information
Safety Statements 24/25
WGK Germany 3
8-10-21
HS Code 29199000
MSDS Information
ProviderLanguage
SigmaAldrich English
ACROS English
4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE Usage And Synthesis
Chemical PropertiesWhite to off-white powder
UsesSubstrate for alkaline and acid phosphatase determinations4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate used in the determination of acid and alkaline phosphatases.
Uses4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
  • enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
  • enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
  • alkaline phosphatase assay
UsesPhosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
General DescriptionChromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
Biochem/physiol Actions4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.
4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE Preparation Products And Raw materials

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