| Hematoxylin Basic information |
| Product Name: | Hematoxylin |
| Synonyms: | GILL 2 METHOD HEMATOXYLIN STAIN;GILL HEMATOXYLIN SOLUTION NO II;GILL'S HEMATOXYLIN NO 2;GILL'S HEMATOXYLIN SOLUTION NO 2;GILLS II HAEMATOXYLIN;HEMATOXYLIN STAIN, GILL 2;HEMATOXYLIN SOLUTION GILL NO 2;HEMATOXYLIN, GILL II |
| CAS: | 517-28-2 |
| MF: | C16H14O6 |
| MW: | 302.28 |
| EINECS: | 208-237-3 |
| Product Categories: | Polymethine;Flavone;Cytology StainsHematology and Histology;marker;Inhibitors;Miscellaneous;Colours, Dyes, Indicators & Pigments;Hematology and Histology;Routine Histology Stains |
| Mol File: | 517-28-2.mol |
| Hematoxylin Chemical Properties |
| Melting point | 200 °C (dec.)(lit.) |
| Boiling point | 363.32°C (rough estimate) |
| density | 1.2514 (rough estimate) |
| vapor pressure | 0-0Pa at 20-25℃ |
| refractive index | 1.4600 (estimate) |
| storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
| solubility | Soluble in 95% ethanol(1 mg/mL). |
| Colour Index | 75290 |
| pka | 6.7(at 25℃) |
| form | Powder/Solid |
| color | Yellow to Brown |
| PH Range | Red (0.0) to yellow (1.0);Pale yellow (5.0) to violet (6.0) |
| Water Solubility | SOLUBLE IN HOT WATER |
| Sensitive | Light Sensitive |
| λmax | 292nm, 445nm, 560nm |
| Merck | 14,4637 |
| BRN | 91399 |
| Stability: | Stability Stable, but may discolour on exposure to light. Incompatible with strong oxidizing agents. |
| Major Application | Plasma displays, textiles, hair dyes, identifying fresh and stale rice, diag-nosing cancer progression, detecting nosing cancer progression, cervical disease, central nervous system malfunctions, detecting genes, breast cancer, collagen in a tissue sample, apoptosis, demyelinating diseases, antigens, treatment of age-related macular degeneration, burns, prostate cancer, diabetesand obesity, viral diseases, neoplasms, peripheral neural and vascular ailments, skin disorders, biotechnological applications, reference standard materials for cytology, histology andimmunohistochemistry |
| InChIKey | WZUVPPKBWHMQCE-UHFFFAOYSA-N |
| LogP | 0.3 at 30℃ and pH6.9 |
| CAS DataBase Reference | 517-28-2 |
| EPA Substance Registry System | Hematoxylin (517-28-2) |
| Safety Information |
| Hazard Codes | Xn,Xi |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 2 |
| RTECS | MH7875000 |
| TSCA | Yes |
| HS Code | 32030019 |
| Hazardous Substances Data | 517-28-2(Hazardous Substances Data) |
| MSDS Information |
| Provider | Language |
|---|---|
| 7,11b-Dihydrobenz[b]indeno[1,2-d]pyran-3,4,6a,9,10(6H)-pentol | English |
| SigmaAldrich | English |
| Hematoxylin Usage And Synthesis |
| Chemical Properties | light brown crystals or powder |
| Uses | Colorant in inks, biological stains. |
| Uses | Hematoxylin and eosin together make up Hematoxylin and eosin stain, one of the most commonly used stains in histology. Metal-haematein complexes are used to stain cell nuclei prior to examination under a microscope. Structures that stain with iron- or aluminum-haematein are often called basophilic, even though the mechanism of the staining is different from that of staining with basic dyes. |
| General Description | White to yellowish crystals that redden on exposure to light. |
| Air & Water Reactions | Soluble in hot water, slightly water soluble in cold . |
| Reactivity Profile | Sensitive to light. . |
| Hazard | May be carcinogenic. |
| Purification Methods | Hematoxylin recrystallises from H2O (as trihydrate) in white-yellow crystals which become red on exposure to light and then melt at 100-120o. It has been recrystallised from Me2CO/*C6H6. It has been recrystallised as well from dilute aqueous NaHSO3 until colourless and is soluble in alkali, borax and glycerol. Store it in the dark below 0o. [Morsingh & Robinson Tetrahedron 26 182 1970, Dann & Hofmann Chem Ber 98 1498 1955, Beilstein 17/8 V 469.] |
| Hematoxylin Preparation Products And Raw materials |
| Raw materials | Ethanol-->Ethyl acetate-->Methanol-->Diethyl ether-->PETROLEUM ETHER-->Acetone |
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