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| BIOTIN HYDRAZIDE Basic information |
| Product Name: | BIOTIN HYDRAZIDE | | Synonyms: | BIOTIN (LONG ARM) HYDRAZIDE;(+)-BIOTIN HYDRAZIDE;BIOTIN HYDRAZIDE;1H-Thieno(3,4-d)imidazole-4-pentanoic acid, hexahydro-2-oxo-, hydrazid e, (3aS,4S,6aR)-;BIOTIN-HYDRAZIDE;Biotin-NHNH2;D-Biotin Hydrazide;1H-Thieno(3,4-D)imidazole-4-pentanoic acid, hexahydro-2-oxo-, hydrazide, (3as-(3aalpha,4beta,6aalpha))- | | CAS: | 66640-86-6 | | MF: | C10H18N4O2S | | MW: | 258.34 | | EINECS: | 613-970-0 | | Product Categories: | Biotinylation Reagents;biotinyl;Biotin Labeling ReagentsGlycobiology;Detection of Biotin Labeled Glycoproteins on Western BlotsAntibody Modification;Labeling with Biotin;BiotinylationReagents;Antibody Labeling;Biotin/Avidin;Glycoprotein Detection;Supplementary Products | | Mol File: | 66640-86-6.mol |
| BIOTIN HYDRAZIDE Chemical Properties |
| Melting point | 245-247 °C | | Boiling point | 633.1±40.0 °C(Predicted) | | density | 1.243±0.06 g/cm3(Predicted) | | storage temp. | 2-8°C | | solubility | DMSO: ≤20 mg/mL | | form | powder | | pka | 13.25±0.35(Predicted) | | color | White to Almost white | | BRN | 28347 | | InChIKey | KOZWHQPRAOJMBN-ZKWXMUAHSA-N | | CAS DataBase Reference | 66640-86-6(CAS DataBase Reference) |
| Safety Statements | 24/25 | | WGK Germany | 3 | | F | 10 | | HS Code | 29349990 |
| BIOTIN HYDRAZIDE Usage And Synthesis |
| Description | Biotin hydrazide is a biotinyl derivative used to label surface functional groups, antibodies, lectins, sugars, nucleic acids, or molecules with free carboxylic or keto groups. Biotin hydrazide is used as a probe for determining protein carbonylation, an irreversible posttranslational modification resulting from the actions of reactive oxygen species or lipid oxidation products. The reaction of this probe is direct and does not require catalysts or reducing agents. | | Chemical Properties | 3.6 | | Uses | aldehyde reactive biotinylation reagent | | Uses | (+)-Biotin hydrazide has been used:- the modification of alginate
- for the labelling of mitochondrial proteins from non-muscle tissues
- as a component of glycoprotein staining solution
- in periodic acid-biotin-hydrazide (PABH) assay for mucins
- for labeling surface functional groups, biologically active molecules such as antibodies, lectins, sugars, nucleic acids or molecules with free carboxylic or keto groups.
- for coupling to glycoproteins through the carbohydrate by hydrazone formation
| | Uses | Biotin Hydrazide is a carbohydrate reactive biotinylation reagent used predominately for labeling monoclonal antibodies, unpaired cytosine residues in nucleic acids and glycoproteins. | | General Description | Biotin hydrazide is a biotinylation reagent used to biotinylate glycoproteins with their sugar moieties. Biotin hydrazide can be used to prepare biotin-conjugated alginate. It can also be used for covalent attachment to PAAc via carbodi-imide cross linking. | | Purification Methods | Wash the hydrazide with H2O, dry it, wash it with MeOH then Et2O, and dry. Recrystallise it from hot H2O (clusters of prisms) [Hofmann et al. J Biol Chem 144 513 1942]. [Beilstein 27 III/IV 7980.] |
| BIOTIN HYDRAZIDE Preparation Products And Raw materials |
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