| DL-Dithiothreitol Basic information |
| Product Name: | DL-Dithiothreitol |
| Synonyms: | DTT-MBG;DTT (RACEMIC);DL-THREO-2,3-DIHYDROXY-1,4-DITHIOLBUTANE;DL-THREO-1,4-DIMERCAPTO-2,3-BUTANEDIOL;DL-DTT;DITHIOTHREITOL, 1,4-;DITT;DITHIOTHRIETOL |
| CAS: | 27565-41-9 |
| MF: | C4H10O2S2 |
| MW: | 154.25 |
| EINECS: | 248-531-9 |
| Product Categories: | Biochemistry;O-Substituted Sugars;Sugar Alcohols;Sugars;Reagents for Electrophoresis |
| Mol File: | 27565-41-9.mol |
| DL-Dithiothreitol Chemical Properties |
| Melting point | 41-44 °C(lit.) |
| Boiling point | 125-130 °C (2 mmHg) |
| density | 1.04 g/mL at 20 °C |
| refractive index | 1.5200 (estimate) |
| Fp | >230 °F |
| storage temp. | 2-8°C |
| solubility | H2O: 50 mg/mL, clear, colorless |
| form | Liquid |
| color | White |
| Water Solubility | Soluble |
| Merck | 3376 |
| CAS DataBase Reference | 27565-41-9(CAS DataBase Reference) |
| EPA Substance Registry System | 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel-(27565-41-9) |
| Safety Information |
| Hazard Codes | Xn |
| Risk Statements | 25-36/37/38-22 |
| Safety Statements | 26-45-36 |
| WGK Germany | 3 |
| RTECS | EK1610000 |
| F | 3-10-16-23 |
| HS Code | 29309090 |
| MSDS Information |
| Provider | Language |
|---|---|
| DL-Dithiothreitol | English |
| SigmaAldrich | English |
| ACROS | English |
| ALFA | English |
| DL-Dithiothreitol Usage And Synthesis |
| Chemical Properties | White solid |
| Uses | Reagent for the quantitative reduction of disulfide group. Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Cleavage of disulfide bonds in proteins. |
| Uses | DTT is a cell permeable protective agent for Thiol groups. |
| Preparation | The preparation of DL-Dithiothreitol is as follows:To a round bottom flask was charged THPP (23mg, 0.11mmol, 1.10eq) and the basic buffer solution [tris-(hydroxymethyl)-aminomethane-CaCl2 based buffer; pH 8.00; 1.0mL]. The reaction mixture was stirred at room temperature for about 5 min and then to this homogenous solution was charged trans-4,5-Dihydroxy-1,2-dithiane (15.2mg, 0.10 mmol, 1.00eq.). The reaction mixture was stirred at room temperature until the reaction was complete (about 30 min). The product was readily identified by 1H-NMR analysis. |
| Purification Methods | Crystallise DTT from ether and sublime it at 37o/0.005mm. It should be stored at 0o. [Beilstein 1 III 2360.] |
| DL-Dithiothreitol Preparation Products And Raw materials |
| Preparation Products | ethyl 2-(2-aminothiazol-5-ylthio)acetate-->DIHYDROLIPOIC ACID |
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