| SEPHADEX G-50 Basic information |
| Description Uses |
| Product Name: | SEPHADEX G-50 |
| Synonyms: | Sephadex G50, BR;PDXTM G.F.;Sephadex(R) G-50 Fine;Sephadex G-50, Medium beads;PDX GF 50 (100-300);PDX GF 50 (50-150);SEPHADEX G-50;SEPHADEX G-50 COARSE |
| CAS: | 9048-71-9 |
| MF: | NULL |
| MW: | 0 |
| EINECS: | |
| Product Categories: | AMEKO;Gel Filtration Media;Sephadex G-50;Gel Filtration Chromatography;Gel Filtration MediaGel Filtration Media;Protein Chromatography;Sephadex;DNA&RNA Purification;Molecular Biology;Reagents |
| Mol File: | Mol File |
| SEPHADEX G-50 Chemical Properties |
| CAS DataBase Reference | 9048-71-9 |
| Safety Information |
| WGK Germany | 3 |
| HS Code | 39140000 |
| MSDS Information |
| Provider | Language |
|---|---|
| SigmaAldrich | English |
| SEPHADEX G-50 Usage And Synthesis |
| Description | Sephadex G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.
|
| Uses | The media used for gel exclusion chromatography include dextran (Sephadex™), polyacrylamide (Bio-Gel P™) and dextran-polyacrylamide (Sephacryl™) and agarose (Sepharose™ and BioGel A™). Each is available with a variety of different ranges of pore size in the beads, permitting separation of macromolecules of different size.
A gel with a smaller range of pore sizes (and hence a smaller range over which it can separate macromolecules on the basis of their size) will give a higher resolution; a gel with a wider range will give lower resolution, but will permit fractionation of a larger range of sizes as an initial step when the approximate molecular mass of the enzyme is not known.
The particle size of the gel beads (the mesh size) also affects resolution; smaller beads permit higher resolution, but a lower flow rate through the column (and hence a slower separation).
The table shows the useful range for the most commonly used gel filtration media - the lower and upper molecular sizes (in kDa) over which they can be used to separate macromolecules. The upper limit is known as the exclusion limit of the gel - the size above which proteins will elute in the void volume of the column.
▼ ▲ lower limit
upper limit
dextran gels
Sephadex G-50
1.5
30
Sephadex G-75
3
80
Sephadex G-100
4
150
Sephadex G-150
5
300
Sephadex G-200
5
600
polyacrylamide gels
Bio-Gel P-10
1.5
20
Bio-Gel P-30
2.5
40
Bio-Gel P-60
3
60
Bio-Gel P-100
5
100
Bio-Gel P-150
15
150
BioGel P-200
30
200
Bio-Gel P-300
60
400
dextran-polyacrylamide gels
Sephacryl S-200
5
250
Sephacryl S-300
10
1500
Sephacryl S-400
20
8000
agarose gels
Sepharose 6B
10
4000
Sepharose 4B
60
20,000
Sepharose 2B
70
40,000
Bio-Gel A-0.5
10
500
Bio-Gel A-1.5
10
1500
Bio-Gel A-5
10
5000
Bio-Gel A-15
40
15,000
Bio-Gel A-50
100
50,000
|
| Uses | Sephadex? G-50 is a gel filtration medium used in affinity chromatography, protein chromatrography and gel filtration chromatography. Fractionation Range (MW)Globular Proteins: 1,500 - 30,000Dextrans: 500 - 10,000 |
| SEPHADEX G-50 Preparation Products And Raw materials |
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