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| 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Basic information |
| 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Chemical Properties |
| 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Usage And Synthesis |
| Uses | Triton? X-100 has been used:- In immunohistochemistry for staining the Flat-mount retinas
- Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
- To permeabilise cells during Immunofluorescent microscopic studies
- As a positive control in LDH assay to determine the cell membrane integrity
- For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
- For the preparation of outer membrane protein exctract
- As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
- In the treatment of tissue sections for Immunofluorescence labeling
| | Uses | 0·3% Triton? X-100 in TBS has been used for permeabilization of retinas before immunostaining. 0.5% Triton X-100 has been used for astrocyte cell lysis. | | Uses | Triton?-X has been used:- In the permeabilization of cells for immunofluorescence staining
- As a component of lysis buffer in western blot analysis
- As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope
| | General Description | Octophenolpoly(ethyleneglycolether)nEspecially used for purification in membrane researchTriton X-100 is a water soluble, non-ionic surfactant. It exhibits a strong hydrogen bonding with water molecule in temperature dependent manner. It has a wide application area such as in isolation and solubilization of protein, DNA extraction and emulsification. | | Biochem/physiol Actions | Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions. | | Safety Profile | Moderately toxic by ingestion.When heated to decomposition it emits acrid smoke andirritating fumes. | | Safety Profile | Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits acridsmoke and irritating fumes. |
| 2-(2-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]ethoxy)ethanol Preparation Products And Raw materials |
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