Other grades of this product :
| BIOTIN-NHS Basic information |
| Product Name: | BIOTIN-NHS | | Synonyms: | (+)-biotin-n-hydroxysuccimide ester;n-hydroxysuccimidobiotin;BIOTIN-OSU: N-HYDROXYSUCCINIMIDOBIOTIN;d-Biotin NHS ester;(2,5-dioxopyrrolidin-1-yl) 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate;5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID 2,5-DIOXO-PYRROLIDIN-1-YL ESTER;D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER;D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER | | CAS: | 35013-72-0 | | MF: | C14H19N3O5S | | MW: | 341.38 | | EINECS: | 609-055-0 | | Product Categories: | biotinyl;BiotinylationReagents;Biotin Derivatives;Cross Linking Reagents;Biotinylation Reagents;PEG | | Mol File: | 35013-72-0.mol |
| BIOTIN-NHS Chemical Properties |
| Melting point | 212-214 °C | | density | 1.45±0.1 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | DMF: ≤50 mg/mL Stable for at least one month in dry DMF | | form | powder | | pka | 13.89±0.40(Predicted) | | color | White to off-white | | BRN | 4720781 | | Stability: | Stable. Incompatible with strong oxidizing agents. | | CAS DataBase Reference | 35013-72-0(CAS DataBase Reference) |
| BIOTIN-NHS Usage And Synthesis |
| Description | N-Hydroxysuccinimide (NHS) activates carboxylic acid groups (on biotin) to facilitate coupling reactions. Biotin-NHS is a compound used to attach biotin to primary amines under alkaline conditions (pH~8-9). For example, lysines on the surface of proteins, including antibodies, are ideal targets for biotinylation with this compound. | | Chemical Properties | White Solid | | Uses | amine reactive biotinylation reagent | | Uses | Biotin-NHS is used in the biotinylation of proteins and peptides. Couples with primary amine in the pH range 6.5-8.5.
| | Uses | Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides. | | Purification Methods | Recrystallise the ester from refluxing isoPrOH and dry it in a vacuum over P2O5 + KOH. [Jasiewicz et al. Exp Cell Biol 100 213 1976.] |
| BIOTIN-NHS Preparation Products And Raw materials |
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