CI 51180

CI 51180
  • CAS No.:3625-57-8
Other grades of this product :
CI 51180 Basic information
Product Name:CI 51180
Synonyms:5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iusulfate(2:1);9-(diethylamino)-5-imino-5h-benzo(a)phenazoxazinsulfate(2:1);Benzo[.alpha.]phenoxazin-7-ium,5-amino-9-(diethylamino)-,sulfate(2:1);nileblueasulphate;BASIC BLUE 12;BASIC BLUE 12 SULFATE SALT;CI NO 51180;CI 51180
CAS:3625-57-8
MF:C20H20N3O5S-
MW:414.46
EINECS:222-832-5
Product Categories:Phenoxazine;marker
Mol File:3625-57-8.mol
CI 51180 Chemical Properties
Melting point >300 °C (dec.)(lit.)
storage temp. room temp
solubility Soluble in water, ethanol
Colour Index 51180
form Powder
color Dark green to black
PH Range10.1(BLUE)-11.1(RED)
Water Solubility Soluble in ethanol, and water (50 g/L) at 25°C.
λmax633 nm
Biological ApplicationsDetecting microorganisms; treating virus infectious diseases; photodynamic therapy
Safety Information
Hazard Codes Xi
Safety Statements 22-24/25
WGK Germany 3
RTECS DJ1931000
HS Code 3212900000
MSDS Information
ProviderLanguage
ACROS English
SigmaAldrich English
ALFA English
CI 51180 Usage And Synthesis
Chemical Propertiesdark green to black powder
UsesNile Blue A is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue
UsesNile Blue A is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. Nile blue A is also useful for staining fats and phospholipids. Dyes and metabolites.
UsesNile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.
General DescriptionNile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.
Biochem/physiol ActionsNile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.
Purification MethodsCrystallise the dye from aqueous AcOH. It has UV: at 630nm (96% aqueous max EtOH) and 635nm (H2O). The betaine has UV: at 513nm (EtOH). [Crossley et al. J Am Chem Soc 74 max 57, 578 1952, Merill & Spencer J Am Chem Soc 70 3683 1948, Beilstein 27 II 457, 27 III/IV 5166.]
CI 51180 Preparation Products And Raw materials
Raw materials5-(diethylamino)-2-nitrosophenol-->1-Naphthylamine

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