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| 5-METHYLCYTOSINE Basic information |
| 5-METHYLCYTOSINE Chemical Properties |
| Melting point | 270°C (rough estimate) | | Boiling point | 232.57°C (rough estimate) | | density | 1.2602 (rough estimate) | | refractive index | 1.6510 (estimate) | | storage temp. | Keep in dark place,Inert atmosphere,Room temperature | | solubility | Methanol (Sparingly, Heated) | | pka | 9.05±0.10(Predicted) | | form | Crystalline | | color | White to Off-White | | Water Solubility | Soluble in water. | | Merck | 14,6050 | | CAS DataBase Reference | 554-01-8(CAS DataBase Reference) |
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ALFA
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| 5-METHYLCYTOSINE Usage And Synthesis |
| Chemical Properties | Crystals in prisms from water. | | Uses | 5-Methylcytosine is used as a marker to protect DNA from being cut by native methylation-sensitive restriction enzymes. It is also employed in the production of 4-amino-5-hydroxy-5-methyl-2-oxo-2,5-dihydro-imidazole-1-carbaldehyde. | | Definition | ChEBI: A pyrimidine that is a derivative of cytosine, having a methyl group at the 5-position. | | Purification Methods | Crystallise it from water (solubility is 3.4%). The hydrochloride has m 299-301o (sintering at 280o) (from aqueous HCl/Me2CO). [Hitchings et al. J Biol Chem 177 537 1949, Cohn J Am Chem Soc 73 1539 1951, Beilstein 25 II 183, 25 III/IV 3727.] |
| 5-METHYLCYTOSINE Preparation Products And Raw materials |
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