Other grades of this product :
| Product Name: | DAF-FM | | Synonyms: | DAF-FM;DIAMINOFLUORESCEIN-FM;4-AMINO-5-METHYLAMINO-2',7'-DIFLUOROFLUORESCEIN;3-AMINO, 4-AMINOMETHYL-2',7'-DIFLUOROFLUORESCEIN;4-Amino-5-methylamino-2′,7′-difluorescein;DAF-FM solution;4-amino-5-methylamino-20,70-difluorodifluorofluorescein;PPRXFLLOWKZHMK-UHFFFAOYSA-N | | CAS: | 254109-20-1 | | MF: | C21H14F2N2O5 | | MW: | 412.34 | | EINECS: | | Product Categories: | Xanthene | | Mol File: | Mol File |
| DAF-FM Chemical Properties |
| Melting point | 265 ℃ | | storage temp. | -20°C | | solubility | DMSO: >1mg/mL | | form | Solid | | pka | 8.19 ± 0.20, most acidic, temperature:
25 °C; 4.23 ± 0.20, most basic(at 25℃) | | color | clear colorless to yellow | | λmax | 487 nm, 495 nm | | Biological Applications | Nitric oxide indicator; detecting microorganisms; measuring Ga i-coupled or Gao-coupled receptors activity |
| Hazard Codes | Xn | | Risk Statements | 22 | | WGK Germany | 3 |
| DAF-FM Usage And Synthesis |
| Uses | DAF-FM is important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. | | Uses | DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells. | | Uses | DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples. | | Biochem/physiol Actions | DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes. |
| DAF-FM Preparation Products And Raw materials |
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