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| CARBOXYPEPTIDASE B Basic information |
| Product Name: | CARBOXYPEPTIDASE B | | Synonyms: | PROTEIN CARBOXYPEPTIDASE B;PROTAMINASE;PEPTIDYL-L-LYSINE[L-ARGININE] HYDROLASE;CARBOXYPEPTIDASE B;CARBOXYPEPTIDASE B, PORCINE PANCREAS;EC 3.4.17.2;IUB: 3.4.17.2;carboxypeptidase B from hog pancreas | | CAS: | 9025-24-5 | | MF: | C31H38N4O7S | | MW: | 610.72102 | | EINECS: | 232-788-9 | | Product Categories: | | Mol File: | 9025-24-5.mol |
| CARBOXYPEPTIDASE B Chemical Properties |
| storage temp. | -20°C | | form | powder | | color | white |
| CARBOXYPEPTIDASE B Usage And Synthesis |
| Uses | The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis. | | Uses | Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis. | | General Description | Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn2+ chelators. | | Biochem/physiol Actions | Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl. |
| CARBOXYPEPTIDASE B Preparation Products And Raw materials |
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