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| ACYL-COENZYME A SYNTHETASE Basic information |
| Product Name: | ACYL-COENZYME A SYNTHETASE | | Synonyms: | EC 6.2.1.3;ACID: COA LIGASE (AMP-FORMING);ACYL-COENZYME A SYNTHETASE;Synthetase, acyl coenzyme A;ACYL-COENZYME A SYNTHETASE FROM PSEUDO-M ONAS SP., ~2.5U/MG;acyl-coenzyme a synthetase from pseudomonas sp.;Acyl-coenzyme A Synthetase [>=8 units/mg];Acyl-CoA Synthetase | | CAS: | 9013-18-7 | | MF: | | MW: | 0 | | EINECS: | 232-747-5 | | Product Categories: | | Mol File: | Mol File |
| ACYL-COENZYME A SYNTHETASE Chemical Properties |
| storage temp. | -20°C | | form | powder | | color | white |
| Safety Statements | 24/25 | | WGK Germany | 3 | | F | 10 | | HS Code | 35079090 |
| ACYL-COENZYME A SYNTHETASE Usage And Synthesis |
| Chemical Properties | White powder | | Uses | Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:- as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)
- in in vitro fatty acylation assays
- in the synthesis of (14C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (3H)Palmitate-CoA
| | General Description | Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily. It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif. | | Biochem/physiol Actions | Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells . | | Purification Methods | Purify the synthase by extraction with sucrose/HCO3 buffer, protamine sulfate precipitation, (NH4)2SO4 (66-65%) fractination (pH 4.35) and a second (NH4)2SO4 (35-60%) fractionation (pH 4.35). It has Km 0.15mM (Vrel 1.0) for octanoate and 0.41mM (Vrel 2.37) for heptanoate. The Km for ATP is 0.5mM, all at pH 9.0 in ethylene glycol buffer at 38o. [Jencks et al. J Biol Chem 204 453 1953, Methods Enzymol 5 467 1962.] |
| ACYL-COENZYME A SYNTHETASE Preparation Products And Raw materials |
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