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| PERTUSSIS TOXIN Basic information |
| Product Name: | PERTUSSIS TOXIN | | Synonyms: | histamine-sensitizingfactor;iap;Protein-based AB5-type exotoxin with five dissimilar subunits: S-1 (M.W. 28 kDa), S-2 (M.W. 23 kDa), S-3 (M.W. 22 kDa), S-4 (M.W. 11.7 kDa) and S-5 (M.W. 9.3 kDa), in a molar ratio of 1:1:1:2:1;Pertussis toxin from Bordetella pertussis,Histamine-sensitizing factor, IAP, Islet Activating Protein, Pertussigen;ANTI-ALKALINE PHOSPHATASE (ALPI) (CENTER antibody produced in rabbit;ANTI-MAGT1 (N-TERM) antibody produced in rabbit;IAG2;Implantation-associated protein | | CAS: | 70323-44-3 | | MF: | NULL | | MW: | 0 | | EINECS: | | Product Categories: | G-Protein | | Mol File: | Mol File |
| PERTUSSIS TOXIN Chemical Properties |
| storage temp. | 2-8°C | | form | lyophilized powder |
| PERTUSSIS TOXIN Usage And Synthesis |
| Uses | The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al. | | General Description | Product is not activated. If activation is required, see Kaslow, et al., for suggested conditions. | | Biochem/physiol Actions | Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels. | | Purification Methods | Purify the toxin by stepwise elution from 3 columns comprising Blue Sepharose, Phenyl Sepharose and hydroxylapatite, and the purity is checked by SDS-PAGE. [Svoboda et al. Anal Biochem 159 402 1986, Biochemistry 21 5516 1982, Biochem J 83 295 1978.] |
| PERTUSSIS TOXIN Preparation Products And Raw materials |
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