Desthiobiotin-PEG3-NHS ester

Overview

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. Please contact us for GMP-grade inquiries.

Application

Products > PEG Linkers > Biotin PEG | Products > PEG Linkers > Biotin PEG > Biotin-PEG-NHS | Products > Biotinylation reagents > Amine Reactive > Biotin-NHS ester

Product Information

• Catalog No.: BP-24457
• CAS No.: 2411681-92-8
• Molecular Formula: C23H38N4O9
• Molecular Weight: 514.6
• Purity: 95%
• Storage: -20°C
• Solubility: Water, DMSO, DCM, DMF
• Availability: In Stock

Source

Original product source