Cy5 DIC18 is a far-red fluorescent carbocyanine dye used for cell membrane labeling both in vivo and in vitro. It diffuses laterally to stain the entire cell, making it suitable as an anterograde and retrograde neuronal tracer. Notably, in intact tissue, the dye does not transfer from labeled to unlabeled cells, although some transfer may occur when the membrane is disrupted (e.g., after sectioning). Initially, the dye is weakly fluorescent, but it becomes more pronounced once incorporated into membranes.
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