Rhodamine 123 (Rh123) is a green-fluorescent cationic xanthene dye used to monitor membrane polarization in mitochondria and bacteria during live cell assays. It responds to the negative potential of the membrane, and its fluorescence intensity decreases with potential loss. Similar to TMRE, Rhodamine 123 is valuable for studying mitochondrial function changes and cell viability in response to various stimuli or pharmaceuticals.
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